By
Bonnie Coblentz MISSISSIPPI
STATE -- A severe infectious bronchitis virus outbreak among
Mississippi broilers was quickly subdued last winter with
the help of improved tests run by Mississippi State
University's College of Veterinary Medicine. Infectious
bronchitis virus causes coughing and sneezing in broilers,
slows the birds' growth and can kill the broiler or cause it
to be condemned at the processing plant. This disease causes
about $1.7 million annual losses in an industry valued at
more than $1.2 billion in 1997. Reagan
Sadler, laboratory manager and poultry pathologist for
Central Industries in Forest, sends all suspected virus
samples he encounters to MSU for testing. "It used
to take weeks to get the results of the tests, but now we're
able to diagnose the problem in about one week," Sadler
said. When an
outbreak of infectious bronchitis virus strikes a company,
between 3 and 8 percent of the broilers are condemned at the
processing plant, Sadler said. Proper vaccination drops that
number down to about 1 percent. Reducing the time it takes
to identify the virus strain and vaccinate against it can
mean a substantial savings for a broiler company. Dr. Roy
Montgomery, CVM associate professor, said the virus is an
on-going problem, but appears periodically as an epidemic.
This year was one of those years, and all Mississippi
broiler companies were affected by it. To date, samples from
more than 160 affected farms have been tested this
year. MSU's
veterinary college helped Mississippi producers react
quickly to the problem. Faculty used two different tests to
quickly determine which strain of the virus was present.
Once the particular strain of the infectious bronchitis
virus was detected, growers could vaccinate incoming flocks
against that strain. Dr.
Frank Austin, veterinary college diagnostic microbiologist,
used monoclonal antibodies in the indirect flourescent
antibody test to detect the presence of the virus by looking
for proteins the virus produced. The test has a four-hour
turnaround from receipt of the specimen to
diagnosis. "This
has dramatically decreased the producers' response time to
the virus," Austin said. "Within a few weeks we can change
the course of an epidemic, where in the past problems might
go on for months." Dr.
Chinling Wang, veterinary college researcher, used the
reverse transcriptase polymerase chain reaction test to
identify the virus. Previously, Wang found a way to shorten
the time it takes to run the RT-PCR test from almost 10 days
to 5 days. "We were
able to quickly identify what kind of virus was involved and
give the poultry companies the information they needed,"
Wang said. The
fluorescent antibody test is quicker and cheaper to run than
is the RT-PCR test, which is more sensitive. Both are
performed for comparison and to confirm the results. In the
height of the outbreak, the lab tested 12 to 15 cases a
week, Wang said. "This is
the first time we've had these two new weapons during an
outbreak," Montgomery said. "Everyone worked on this
together to solve the problem quickly." Not all
states have diagnostic labs to support their poultry
industry and Mississippi has only recently gained this
ability, Montgomery said. The seven people who work to
diagnose this virus in the state's poultry industry are
Montgomery, Wang, Austin, Sadler, and Drs. Bob Keirs, Danny
Magee and Dr. Sue Ann Hubbard, all with MSU's veterinary
college. Released:
Aug. 24, 1998
Animal
Health
Vet College
Helped Stop Big Outbreak
Contact: Dr. Roy Montgomery, (601) 325-1279
Visit: DAFVM
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Last Modified: Friday, 17-Aug-07 14:30:11
URL: http://msucares.com/news/print/cvm/cvm98/980824rm.htm
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