By
Bonnie Coblentz MISSISSIPPI
STATE -- The fight against a costly virus affecting the
state's billion dollar poultry industry recently got a boost
when researchers improved the testing procedure. Dr.
Chinling Wang, a researcher with Mississippi State
University's College of Veterinary Medicine, has found a way
to shorten the time needed to run the RT-PCR (reverse
transcriptase polymerase chain reaction) test. This test
accurately and now quickly detects and identifies infectious
bronchitis virus in poultry. Infectious
bronchitis is a major problem in the poultry industry around
the state and country, said Dr. Tom Smith, MSU extension
poultry specialist. The poultry industry loses an estimated
$1.7 million yearly to the virus. Smith
said about 1.5 percent of the birds produced in Mississippi
are condemned at the processing plants, and of these, about
15 percent are due to respiratory problems such as
infectious bronchitis. In an industry worth $1.14 billion in
1996, that represents about $1.7 million lost to the
virus. "Any
test that more accurately and quickly identifies this virus
so the birds can be vaccinated properly saves the industry
money," he said. Wang's
research is doing just that. "It used
to take six to seven days to run the PCR test, but we've
gotten it down to three to four days," Wang said. "The
poultry industry always wants to know the results quickly,
and this test allows for a faster response." The PCR
test is also more sensitive than the IFA test (indirect
fluorescent antibody) used by some to detect the virus. With
more than 20 known strains of infectious bronchitis, the IFA
test can only detect three types, even if more are present.
The PCR test can identify most strains. Wang
said all broilers in the United States are vaccinated
against infectious bronchitis and other diseases, but some
still get infectious bronchitis. "The
virus mainly causes respiratory problems where the bird
sneezes and coughs," Wang said. "But once the bird has
bronchitis, it's easier to get a secondary infection, such
as E. coli, which can lead to airsaculitis." Birds
with airsaculitis are condemned at the processing plant,
causing the producers to lose money. Even when airsaculitis
does not develop, infectious bronchitis can be deadly and
lowers egg production, Wang said. To run a
PCR test, a sample of the virus must be taken and injected
in an egg so the virus can replicate. The genes of the virus
are then taken from the egg and amplified so they can be
identified. Once
identified, the results can be used to recommend the
appropriate vaccine for this particular strain of the
virus. Industry
losses can be reduced if the proper vaccine are given to
infected birds. The CVM
is the only location in Mississippi that offers the PCR test
for infectious bronchitis, Wang said. Many in the poultry
industry request the PCR test, but until last year when Wang
started her research, testing was done out of
state. Wang's
research is focusing on finding what strain of the
infectious bronchitis virus is prevalent in Mississippi
broilers. She is trying to determine how long the virus
stays in the bird and what tissue works best for diagnosis.
She is also working to determine why so many types of
infectious bronchitis exist. Released:
Feb. 3, 1997
Animal
Health
CVM Research Aids
Poultry Industry
Contact: Dr. Chinling Wang, (601) 325-1683
Visit: DAFVM
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Last Modified: Friday, 17-Aug-07 14:30:07
URL: http://msucares.com/news/print/cvm/cvm97/970203cw.htm
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